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Antimicrobial and inflammatory effects of bacteriocins
Produced and purified from multidrug resistance Staphylococcus aureus and Pseudomonas aeruginosa isolated from clinical samples
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One hundred fifty clinical samples (burns, wounds, urine, nasal and ear swabs) were collected from patients of Al-Yarmouk Hospital and Teaching Baghdad Hospital during the period from November / 2015 to January / 2016. Multi drug resistant strains are serious problems in public health worldwide. So, the continueous development of new classes of antimicrobial agents has become of increasing importance for medicine. Therefore was aimed to produce of Crude and purified bacteriocins from antibiotic resistant bacteria (G-ve MRSA and G+ve P. aeruginosa) isolated from clinical samples and determine the optimization conditions for production of bacteriocin and their purification and characterization. In addition to characterize their inhibitory activity in-vitro and in- vivo. Cultural and morphological characteristice examination, biochemical tests were conducted and confirmed the diagnosis by API20E and Staph API and antibiotics sensitivity test confirmed by Vitek-2 system.
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