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Method For Simultaneous Estimation Of Glucosamine And Boswellic Acids

Name: Method For Simultaneous Estimation Of Glucosamine And Boswellic Acids
Brand: LAP Lambert Academic Publishing
SKU: 15664917
Price: 35.49 EUR
Availability: InStock
Author: Badmanaban Ramalingam
ISBN: 9783330015289

Development and Validation of Analaytical method by HPTLC

Badmanaban Ramalingam

Vala Sanjaykumar Govindbhai

Prasanth Balakrishnapillai

Idioma

Inglés

Libro Tapa blanda

Código Libristo: 15664917

Editores LAP Lambert Academic Publishing, diciembre 2016

Objective: The objective is to develop and validate simple, accurate, sensitive and rapid , HPTLC me... Descripción completa

Código Libristo: 15664917

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Objective: The objective is to develop and validate simple, accurate, sensitive and rapid , HPTLC method for simultaneous estimation of Glucosamine (GLU) and ß-Boswellic acids (BAs) in their combined tablet dosage form. Experimental Work: In HPTLC method, chromatographic separation was carried out on TLC plate pre-coated with silica gel 60F254 using mobile phase-A,Toluene-Ethylacetate (2.5:1.5 v/v) for 11-KBA and A-11-KBA,scanning the plate at 260 nm [scan-1] and mobile phase-B, 1-Butanol -Water-Glacial acetic acid (3:1:1, v/v/v) for GLU ,derivatized the plate by Ninhydrin reagent and scanning the plate at 485 nm[scan-2]. Results: The HPTLC method was found to be linear over the range of 6250-31250 , 300-1500 and 150-750 ng/band for GLU,11-KBA and A-11-KBA , respectively.In this method, % RSD of assay determination of both drugs was found to be less than 2.0 and mean % recoveries were found between 98-101 %. Conclusion: The method was found to be precise as % RSD of assay in all cases was less than 2.0. High percentage recoveries obtained in this method indicate that the method is accurate. There was no interference from any excipients in the determination of drugs in tablets.