Ribosome Biogenesis and Function

This open access volume presents a comprehensive collection of state-of-the-art methodologies for investigating ribosome biogenesis and function. The chapters cover a broad spectrum of experimental approaches, ranging from the visualization of the nucleolus-the cell's ribosome factory-using cutting-edge imaging techniques, such as ten-fold expansion microscopy and digital holographic microscopy, to the analysis of pre-rRNA processing and RNA modifications at single-nucleotide resolution using long-read sequencing approaches, including NanoRibolyzer.

Ribosome function is explored through a diverse array of complementary methodologies, including polysome profiling across different experimental models, various forms of ribosome profiling (Ribo-seq), biochemical assays of translation in vitro, and the visualization of protein synthesis in living cells at single-molecule resolution.

Written in the highly successful Methods in Molecular Biology series format, each chapter provides an introduction to the underlying scientific principles, detailed lists of required materials and reagents, step-by-step protocols designed to ensure reproducibility, and practical advice on troubleshooting and avoiding common pitfalls.

Combining innovative approaches with established methodologies, Ribosome Biogenesis and Function: Methods and Protocols offers a comprehensive and practical laboratory resource for both newcomers to the field and experienced investigators seeking to expand their experimental repertoire.

This publication is based upon work carried out within the framework of COST Action TRANSLACORE (CA21154), supported by COST (European Cooperation in Science and Technology). COST is a funding organization dedicated to fostering research and innovation networks across Europe. By connecting researchers and facilitating the exchange of knowledge, expertise, and ideas, COST Actions promote scientific collaboration, advance research, and support career development and innovation.